A Text-Book of Medical Bacteriology by R. W. Fairbrother (Auth.)

By R. W. Fairbrother (Auth.)

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Extra resources for A Text-Book of Medical Bacteriology

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A total count. 1. Viable Count. The number of living bacteria in a suspension is determined by plating a standard amount of serial decimal dilutions of the suspension. Dilutions in saline up to 1/107 or 1/108 of the original suspension are made ; a fresh pipette must be used for each bacterial transfer. c. of each dilution is placed in a Petri dish, melted agar, cooled to 50°-55° C , is added and the contents of the plate mixed by gentle agitation. After incubation at the optimum temperature for 24-48 hours, the total number of colonies on plates containing a countable number is obtained and an average made after the dilutions have been adjusted.

Infra-red and red rays, have very little bactericidal action, while the ultra-violet rays are those mainly responsible for the destruction of bacteria. Moreover, this destructive action is not restricted to bacteria, but is exercised on all unicellular organisms and also the body-cells. The power of penetration of these rays is, however, low, so t h a t the action is limited mainly to the surface growth. The mode of action is THE DESTRUCTION OF BACTERIA 55 not definitely known, b u t the indications are t h a t it is more a chemical than a physical process.

This method is used largely for the isolation of anaerobic spore-bearing organisms from contaminated material. 7. Animal Inoculation. In some cases the most satisfactory method of isolating bacteria is by the inoculation of susceptible laboratory animals, such as mice or guinea-pigs. This method is used extensively for the isolation of the tubercle bacillus from milk, pus, etc. ; it is also employed for isolation of the pneumococcus from sputum in cases of pneumonia, and the anthrax bacillus from contaminated material, such as shaving-brushes.

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