42 Movement Form by Tai Chi Yang

By Tai Chi Yang

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When organ culture is used for corneal storage, bacterial septicemias are not contraindications to corneal tissue transplantation. In fact, aqueous humor contamination is rare during bacterial septicemias, and organ culture permits detection of contaminated corneas before delivering tissue for transplantation [26]. Conversely, if hypothermic storage is to be used, bacterial septicemias have to be considered as contraindications to transplantation. Transmission of bacteria and fungi by corneal transplantation has been reported, and it may result in postoperative keratitis or endophthalmitis [27].

On the one hand the microbiological safety is affected by decontamination and microbiological testing procedures in the bank. On the other hand additional measures may be taken in the clinic. According to EU regulations, the air quality during the processing of the cornea is considered a key factor in tissue processing. In the CBA the handling of the corneoscleral button using aseptic techniques is performed under aseptic conditions provided by a laminar airflow cabinet with an air quality comparable to good manufacturing practice (GMP) grade A.

36 Meier FM, Tschanz SA, Ganzfried R, Epstein D: A comparative assessment of endothelium from pseudophakic and phakic donor corneas stored in organ culture. Br J Ophthalmol 2002;86:400–403. Vincent M. Borderie, MD, PhD Centre Hospitalier National d’Ophtalmologie des XV–XX 28, rue de Charenton FR–75012 Paris (France) Tel. fr 30 Borderie Bredehorn-Mayr T, Duncker GIW, Armitage WJ (eds): Eye Banking. Dev Ophthalmol. Basel, Karger, 2009, vol 43, pp 31–46 Organ Culture Preservation for Corneal Tissue Technical and Quality Aspects E.

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